Responses of cutaneous C-fiber afferents and spinal microglia after hindlimb cast immobilization in rats.
J Physiol Sci
; 71(1): 19, 2021 Jun 23.
Article
en En
| MEDLINE
| ID: mdl-34162322
ABSTRACT
Previous studies have shown that persistent limb immobilization using a cast increases nociceptive behavior to somatic stimuli in rats. However, the peripheral neural mechanisms of nociception remain unclear. Using single-fiber electrophysiological recordings in vitro, we examined the general characteristics of cutaneous C-fiber afferents in the saphenous nerve and their responsiveness to mechanical and heat stimuli in a rat model of immobilization-induced pain by subjecting the rats to hindlimb cast immobilization for 4 weeks. The mechanical response of C-fibers appeared to increase in the model; however, statistical analysis revealed that neither the response threshold nor the response magnitude was altered. The general characteristics and heat responses of the C-fibers were not altered. The number of microglia and cell diameters significantly increased in the superficial dorsal horn of the lumbar spinal cord. Thus, activated microglia-mediated spinal mechanisms are associated with the induction of nociceptive hypersensitivity in rats after persistent cast immobilization.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Piel
/
Médula Espinal
/
Moldes Quirúrgicos
/
Microglía
/
Fibras Nerviosas Amielínicas
/
Miembro Posterior
/
Inmovilización
/
Neuronas Aferentes
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Physiol Sci
Asunto de la revista:
FISIOLOGIA
/
TRAUMATOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Japón