Ajuba transactivates N-cadherin expression in colorectal cancer cells through interaction with Twist.
J Cell Mol Med
; 25(16): 8006-8014, 2021 08.
Article
en En
| MEDLINE
| ID: mdl-34173718
ABSTRACT
Ajuba is a multiple LIM domain-containing protein and functions as a transcriptional coregulator to modulate many gene expressions in various cellular processes. Here, we describe that the LIM domain of Ajuba interacts with Twist, and the Twist box is a pivotal motif for the interaction. Biologically, Ajuba enhances transcription of target gene N-cadherin as an obligate coactivator of Twist. The enhancement is achieved by binding to the E-box element within N-cadherin promoter as revealed by luciferase reporter and chromatin immunoprecipitation assays. Mechanistic investigation demonstrates that Ajuba recruits CBP and Twist to form a ternary complex at the Twist target promoter region and concomitantly enhances histone acetylation at these sites. These findings identify that Twist is a new interacting protein of Ajuba and Ajuba/Twist/CBP ternary complex may be a potential treatment strategy for Twist-related tumour metastasis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Sialoglicoproteínas
/
Proteínas Nucleares
/
Neoplasias Colorrectales
/
Antígenos CD
/
Cadherinas
/
Regulación de la Expresión Génica
/
Regiones Promotoras Genéticas
/
Proteína 1 Relacionada con Twist
/
Proteínas con Dominio LIM
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Cell Mol Med
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
China