Constructing a gnotobiotic mouse model with a synthetic human gut microbiome to study host-microbe cross talk.
STAR Protoc
; 2(2): 100607, 2021 06 18.
Article
en En
| MEDLINE
| ID: mdl-34179836
ABSTRACT
Reproducible in vivo models are necessary to address functional aspects of the gut microbiome in various diseases. Here, we present a gnotobiotic mouse model that allows for the investigation of specific microbial functions within the microbiome. We describe how to culture 14 different well-characterized human gut species and how to verify their proper colonization in germ-free mice. This protocol can be modified to add or remove certain species of interest to investigate microbial mechanistic details in various disease models. For complete details on the use and execution of this protocol, please refer to Desai et al. (2016).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Interacciones Huésped-Patógeno
/
Microbioma Gastrointestinal
Límite:
Animals
/
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Luxemburgo