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Constructing a gnotobiotic mouse model with a synthetic human gut microbiome to study host-microbe cross talk.
Steimle, Alex; De Sciscio, Alessandro; Neumann, Mareike; Grant, Erica T; Pereira, Gabriel V; Ohno, Hiroshi; Martens, Eric C; Desai, Mahesh S.
Afiliación
  • Steimle A; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette 4354, Luxembourg.
  • De Sciscio A; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette 4354, Luxembourg.
  • Neumann M; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette 4354, Luxembourg.
  • Grant ET; Faculty of Science, Technology and Medicine, University of Luxembourg, Esch-sur-Alzette 4365, Luxembourg.
  • Pereira GV; Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette 4354, Luxembourg.
  • Ohno H; Faculty of Science, Technology and Medicine, University of Luxembourg, Esch-sur-Alzette 4365, Luxembourg.
  • Martens EC; University of Michigan Medical School, Ann Arbor, MI 48109, USA.
  • Desai MS; RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa 230-0045, Japan.
STAR Protoc ; 2(2): 100607, 2021 06 18.
Article en En | MEDLINE | ID: mdl-34179836
ABSTRACT
Reproducible in vivo models are necessary to address functional aspects of the gut microbiome in various diseases. Here, we present a gnotobiotic mouse model that allows for the investigation of specific microbial functions within the microbiome. We describe how to culture 14 different well-characterized human gut species and how to verify their proper colonization in germ-free mice. This protocol can be modified to add or remove certain species of interest to investigate microbial mechanistic details in various disease models. For complete details on the use and execution of this protocol, please refer to Desai et al. (2016).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interacciones Huésped-Patógeno / Microbioma Gastrointestinal Límite: Animals / Humans Idioma: En Revista: STAR Protoc Año: 2021 Tipo del documento: Article País de afiliación: Luxemburgo

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Interacciones Huésped-Patógeno / Microbioma Gastrointestinal Límite: Animals / Humans Idioma: En Revista: STAR Protoc Año: 2021 Tipo del documento: Article País de afiliación: Luxemburgo