Evaluation of Rapid IgG4 Test for Diagnosis of Gnathostomiasis.
Korean J Parasitol
; 59(3): 257-263, 2021 Jun.
Article
en En
| MEDLINE
| ID: mdl-34218597
ABSTRACT
Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Paragonimiasis
/
Gnathostomiasis
/
Gnathostoma
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Korean J Parasitol
Asunto de la revista:
PARASITOLOGIA
Año:
2021
Tipo del documento:
Article