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MiR-622 acts as a tumor suppressor to induce cell apoptosis and inhibit metastasis in human prostate cancer.
Targhazeh, Niloufar; Yousefi, Bahman; Asghari, Samira; Mohammadnejhad, Reza; Mansouri, Parinaz; Valizadeh, Amir.
Afiliación
  • Targhazeh N; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Yousefi B; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Asghari S; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Mohammadnejhad R; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Mansouri P; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Valizadeh A; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Andrologia ; 53(9): e14174, 2021 Oct.
Article en En | MEDLINE | ID: mdl-34231241
ABSTRACT
Growing evidence indicating the critical modulator roles of microRNAs (miRNAs) involved in prostate cancer (PCa) metastasis that holds great promise as therapeutic targets. Herein, we transfected the miR-622 mimic into PC3 cells and evaluated the effects of this interference on these tumour cells' growth and the expression of specific metastatic genes. Transfecting of miR-622 mimic and inhibitor, negative control (NC) inhibitor and NC was established using Lipofectamine 2000. The mRNA levels of miR-622 and metastatic genes were evaluated using the qRT-PCR and Western blot. Cytotoxic effects of miR-622 were assessed by MTT. Apoptosis was detected using an ELISA cell death assay kit. miR-622 is down-regulated in PC3 cells. As expected, cell viability effects after transfection were described as miR-622 inhibitor >NC and NC inhibitor >miR-622 mimic (p < .01). Importantly, we showed that transfected miR-622 mimic could enhance the apoptosis of PC3 cells, while transfected miR-622 inhibitor could decrease cell apoptosis (p < .01). Furthermore, miR-622 overexpression could increase significantly down-regulated the MMP2, MMP9, CXCR-4, c-Myc and K-Ras expression levels. Findings demonstrate a novel mechanism by which miR-622 modulates PCa cells' metastasis by targeting metastatic genes. These data confirm the tumour-suppressive function of miR-622 in PCa cells by enhancing apoptosis and reducing metastasis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / MicroARNs Límite: Humans / Male Idioma: En Revista: Andrologia Año: 2021 Tipo del documento: Article País de afiliación: Irán

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Próstata / MicroARNs Límite: Humans / Male Idioma: En Revista: Andrologia Año: 2021 Tipo del documento: Article País de afiliación: Irán