Melatonin prevents periimplantitis via suppression of TLR4/NF-κB.
Acta Biomater
; 134: 325-336, 2021 10 15.
Article
en En
| MEDLINE
| ID: mdl-34271168
ABSTRACT
Periimplantitis, which is characterized by periimplant mucositis and alveolar bone resorption, significantly shortens the service life of dental implants. Melatonin is well-known for its anti-inflammatory and osteoprotective activities. Nevertheless, the effects and mechanisms of melatonin to prevent periimplantitis remain unknown. In this study, the lipopolysaccharide-induced periimplantitis model was established after the titanium implants were osseointegrated, and the rats received daily administrations of melatonin. The gingival fibroblasts and osteoclasts/osteoblasts were also co-cultured to simulate the inflammatory environment in vitro. We found that prophylactic administration of melatonin decreased proinflammatory cytokine levels and osteoclast numbers, attenuated alveolar bone resorption, and reduced the incidence of periimplantitis in vivo. Furthermore, melatonin suppressed osteoclastic formation and function in the inflammatory co-culture environment, while melatonin promoted osteoblastic differentiation and function in the in vitro model. Mechanistically, melatonin reduced TLR4 protein levels, and inhibited activation of NF-κB to downregulate the levels of TNF, IL-1ß, and IL-6. These data showed that melatonin was a potent agent to prevent periimplantitis through inhibiting TLR4/NF-κB signaling. Our findings provide a novel strategy to prevent periimplantitis, and expand the applications of melatonin. STATEMENT OF SIGNIFICANCE:
Dental implants have become the first choice for restoring partial and full edentulism, but its service life is seriously affected by periimplantitis. Exploration of novel and effective approaches to prevent periimplantitis is an important and urgent need. In the present study, we have reported for the first time that prophylactic administration of melatonin delayed the occurrence and reduced the incidence of periimplantitis by decreasing proinflammatory cytokine levels, inhibiting osteoclastogenesis, and promoting osteogenesis. The study is expected to have an important significance on the prevention of periimplantitis.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Resorción Ósea
/
Pérdida de Hueso Alveolar
/
Periimplantitis
/
Melatonina
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Acta Biomater
Año:
2021
Tipo del documento:
Article