Quantitative phase microscopy monitors subcellular dynamics in single cells exposed to nanosecond pulsed electric fields.
J Biophotonics
; 14(10): e202100125, 2021 10.
Article
en En
| MEDLINE
| ID: mdl-34291579
ABSTRACT
A substantial body of literature exists to study the dynamics of single cells exposed to short duration (<1 µs), high peak power (~1 MV/m) transient electric fields. Much of this research is limited to traditional fluorescence-based microscopy techniques, which introduce exogenous agents to the culture and are only sensitive to a single molecular target. Quantitative phase imaging (QPI) is a coherent imaging modality which uses optical path length as a label-free contrast mechanism, and has proven highly effective for the study of single-cell dynamics. In this work, we introduce QPI as a useful imaging tool for the study of cells undergoing cytoskeletal remodeling after nanosecond pulsed electric field (nsPEF) exposure. In particular, we use cell swelling, dry mass and disorder strength measurements derived from QPI phase images to monitor the cellular response to nsPEFs. We hope this demonstration of QPI's utility will lead to a further adoption of the technique for the study of directed energy bioeffects.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Electricidad
/
Microscopía
Idioma:
En
Revista:
J Biophotonics
Asunto de la revista:
BIOFISICA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos