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Endogenous Enzymatic Activity of Primary and Permanent Dentine.
Maravic, Tatjana; Breschi, Lorenzo; Paganelli, Federica; Bonetti, Giulio Alessandri; Martina, Stefano; Di Giorgio, Gianni; Bossù, Maurizio; Polimeni, Antonella; Checchi, Vittorio; Generali, Luigi; Tay, Franklin R; Cadenaro, Milena; Mazzoni, Annalisa.
Afiliación
  • Maravic T; Department of Biomedical and Neuromotor Sciences, University of Bologna-Alma Mater Studiorum, 40125 Bologna, Italy.
  • Breschi L; Department of Biomedical and Neuromotor Sciences, University of Bologna-Alma Mater Studiorum, 40125 Bologna, Italy.
  • Paganelli F; Department of Biomedical and Neuromotor Sciences, University of Bologna-Alma Mater Studiorum, 40125 Bologna, Italy.
  • Bonetti GA; Department of Biomedical and Neuromotor Sciences, University of Bologna-Alma Mater Studiorum, 40125 Bologna, Italy.
  • Martina S; Department of Medicine, Surgery and Dentistry, University of Salerno, 84081 Baronissi (Salerno), Italy.
  • Di Giorgio G; Department of Oral and Maxillofacial Science, "Sapienza" University of Rome, 00161 Rome, Italy.
  • Bossù M; Department of Oral and Maxillofacial Science, "Sapienza" University of Rome, 00161 Rome, Italy.
  • Polimeni A; Department of Oral and Maxillofacial Science, "Sapienza" University of Rome, 00161 Rome, Italy.
  • Checchi V; Department of Surgery, Medicine, Dentistry and Morphological Sciences with Transplant Surgery, Oncology and Regenerative Medicine Relevance, University of Modena and Reggio Emilia, 41124 Modena, Italy.
  • Generali L; Department of Surgery, Medicine, Dentistry and Morphological Sciences with Transplant Surgery, Oncology and Regenerative Medicine Relevance, University of Modena and Reggio Emilia, 41124 Modena, Italy.
  • Tay FR; The Dental College of Georgia, Augusta University, Augusta, GA 30912, USA.
  • Cadenaro M; Department of Medical Sciences, University of Trieste, 34125 Trieste, Italy.
  • Mazzoni A; Institute for Maternal and Child Health-IRCCS "Burlo Garofolo", 34137 Trieste, Italy.
Materials (Basel) ; 14(14)2021 Jul 20.
Article en En | MEDLINE | ID: mdl-34300961
Matrix metalloproteinases (MMPs) play an important role in tooth development and influence caries development and hybrid layer degradation. Literature is scant on the differences in the activity of MMPs between primary and permanent dentine. Accordingly, the aim of the present study was to investigate endogenous gelatinolytic activity in primary and permanent dentine. Separate batches of dentine powder were obtained from intact human primary and permanent molars (n = 6). Each batch was divided in two subgroups: (1) mineralised; and (2) demineralised with 10% H3PO4. After protein extraction, gelatine zymography was performed. Furthermore, in situ zymography was performed on dentine sections of the same groups (n = 3). The slices were polished, covered with fluorescein-conjugated gelatine and evaluated using a confocal microscope. In situ zymography data were analysed using two-way analysis of variance and post hoc Holm-Sidák statistics (α = 0.05). Primary dentine showed poorly defined bands in the zymograms that vaguely corresponded to the pro-form and active form of MMP-2 and the pro-form of MMP-9. In permanent dentine, demineralised powder demonstrated stronger gelatinolytic activity than mineralised powder. In situ zymography identified stronger enzymatic activity in primary etched dentine (p < 0.05). Stronger enzymatic activity recorded in primary dentine may be related to the differences in morphology and composition between primary and permanent dentine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Materials (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Materials (Basel) Año: 2021 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Suiza