Multiplexed Fourier Transform Infrared and Raman Imaging.
Methods Mol Biol
; 2350: 299-312, 2021.
Article
en En
| MEDLINE
| ID: mdl-34331293
ABSTRACT
Infrared (IR) and Raman spectroscopies are being increasingly employed for the label-free analysis of biochemical samples. Both are vibrational imaging techniques, but they provide complementary information about the chemical composition of the sample, and thus the integration of Raman and IR images leads to a comprehensive understanding of the samples. Here, we summarize the steps needed for performing multiplexed infrared and Raman imaging, identifying and overcoming the two main challenges first, the technical difficulties caused by the incompatibilities of the techniques and, second, the necessity to extract the information from the large number of vibrational variables found in both IR and Raman spectra.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría Raman
/
Espectroscopía Infrarroja por Transformada de Fourier
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
España