ESRP1 as a prognostic factor of non-small-cell lung cancer is related to the EMT transcription factor of Twist.
Thorac Cancer
; 12(18): 2449-2457, 2021 09.
Article
en En
| MEDLINE
| ID: mdl-34342121
ABSTRACT
OBJECTIVE:
Non-small-cell lung cancer (NSCLC) is one of the most common fatal cancers in the world. Although the treatment of NSCLC has been significantly improved, there is still an unmet need to identify novel targets for developing therapeutic agents and diagnostic/prognostic markers. The aim of this study is explore the role and underlying mechanism of the epithelial splicing regulatory protein (ESRP1) in the development and progression of NSCLC.METHODS:
A total of 115 participants, 65 cases of NSCLC, 20 cases of precancerous lesions, and 30 cases of benign lung nodules, were included in this study. The expressions of ESRP1 and related transcription factor Twist in enrolled lung tissues were evaluated by histochemistry and immunohistochemistry assay. The survival analysis and related prognosis factors were evaluated by the Kaplan-Meier curve and Cox regression. In addition, the expression of ESRP1 and epithelial-mesenchymal transition (EMT)related transcription factor Twist and EMT markers E-cadherin and N-cadherin were ascertained by immunohistochemical and immunoblotting assay on A549 lung adenocarcinoma cell lines that were exposed to transforming growth factor ß1 (TGFß1).RESULTS:
Compared with normal lung tissues, the abundance of ESRP1 protein was significantly increased in precancerous lesions and lung cancer. Correlation analysis demonstrated that ESRP1 was an independent prognostic factor in NSCLC. The expression of ESRP1 and Twist was positively correlated in lung tissues (r = 0.285, p < 0.001). In vitro analysis further showed that TGFß1 could upregulate the expression of EMT transcription factor Twist while downregulating ESRP1.CONCLUSIONS:
Our data suggest that the aberrant expression of ESRP1 is an early event in the development of NSCLC. The ESRP1 could serve as a prognostic biomarker for NSCLC, particularly when combined with Twist. The Twist negatively regulated the expression of ESRP1, emphasizing the role of the TGFß/ESRP1 pathway in the development of NSCLC, which warrants further investigation.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Unión al ARN
/
Carcinoma de Pulmón de Células no Pequeñas
/
Transición Epitelial-Mesenquimal
/
Factores de Transcripción Twist
/
Neoplasias Pulmonares
Tipo de estudio:
Prognostic_studies
Límite:
Adult
/
Aged
/
Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Thorac Cancer
Año:
2021
Tipo del documento:
Article
País de afiliación:
China