Apoptosis in the late replication phase of Bovine alphaherpesvirus 1 in experimentally infected calves.
Braz J Microbiol
; 52(4): 2529-2534, 2021 Dec.
Article
en En
| MEDLINE
| ID: mdl-34355356
ABSTRACT
Bovine alphaherpesvirus 1 (BoHV-1) is a pathogen causing respiratory and reproductive clinical signs in cattle. Infected animals may develop rhinotracheitis, vulvovaginitis, balanoposthitis, and abortion. Viral latency is generally established in neuronal ganglia simultaneously to a decrease in both genes or genome expression and viral replication. Under stressful conditions, infection is reactivated leading to viral replication and the manifestation of clinical signs. In this study, we evaluated both viral reactivation and apoptosis in trigeminal ganglia cells as BoHV-1 progressed from the latent to the acute phase of infection after dexamethasone administration in experimentally infected calves. To test ganglia cell death as a consequence of BoHV-1 infection, we stained the BoHV-1 samples with TUNEL after the viral shedding by the calves. RT-qPCR of apoptotic genes was also performed, showing the upregulation of the caspase 8 gene in the trigeminal ganglia from cattle experimentally infected with BoHV-1. These results showed the occurrence of apoptosis in ganglion cells of calves infected by BoHV-1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de los Bovinos
/
Apoptosis
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Infecciones por Herpesviridae
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Herpesvirus Bovino 1
Límite:
Animals
Idioma:
En
Revista:
Braz J Microbiol
Año:
2021
Tipo del documento:
Article
País de afiliación:
Brasil