Detection of Long Noncoding RNA Expression by Real-Time PCR.
Methods Mol Biol
; 2372: 35-42, 2021.
Article
en En
| MEDLINE
| ID: mdl-34417741
ABSTRACT
Recent advances in sequencing technology and bioinformatic analysis promote the identification of long noncoding RNA (lncRNA), a novel form of untranslated RNA transcript. Long coding RNA has been extensively investigated for its fundamental biological functions in a broad spectrum of pathogenetic and therapeutic studies. The level of lncRNA expression varies among different tissue types and contexts. Therefore, a quantitative method to accurately measure lncRNA expression is highly desired. Real-time PCR analysis enables us to conveniently determine the identity and abundance of lncRNA in a time-efficient manner. Here, we introduce how to use real-time PCR to analyze and quantify a defined lncRNA.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Reacción en Cadena en Tiempo Real de la Polimerasa
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2021
Tipo del documento:
Article
País de afiliación:
China