Validation of gene editing efficiency with CRISPR-Cas9 system directly in rat zygotes using electroporation mediated delivery and embryo culture.

Challa, Anil K; Stanford, Denise; Allen, Antonio; Rasmussen, Lawrence; Amanor, Ferdinand K; Raju, S Vamsee

Challa, Anil K; Stanford, Denise; Allen, Antonio; Rasmussen, Lawrence; Amanor, Ferdinand K; Raju, S Vamsee.

Afiliación

Challa AK; Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
Stanford D; Cystic Fibrosis Research Center, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Allen A; Cystic Fibrosis Research Center, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Rasmussen L; Cystic Fibrosis Research Center, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Amanor FK; Cystic Fibrosis Research Center, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.
Raju SV; Cystic Fibrosis Research Center, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.

MethodsX ; 8: 101419, 2021.

Article en En | MEDLINE | ID: mdl-34430314

Palabras clave

Chrna7; Heteroduplex mobility assay (HMA); Homology-driven repair (HDR); Indels

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Aspecto: Implementation_research Idioma: En Revista: MethodsX Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos

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