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InVision: An optimized tissue clearing approach for three-dimensional imaging and analysis of intact rodent eyes.
Gurdita, Akshay; Nickerson, Philip E B; Pokrajac, Neno T; Ortín-Martínez, Arturo; Samuel Tsai, En Leh; Comanita, Lacrimioara; Yan, Nicole E; Dolati, Parnian; Tachibana, Nobuhiko; Liu, Zhongda C; Pearson, Joel D; Chen, Danian; Bremner, Rod; Wallace, Valerie A.
Afiliación
  • Gurdita A; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, 60 Leonard Avenue, Toronto, ON M5T 2S8, Canada.
  • Nickerson PEB; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Pokrajac NT; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, 60 Leonard Avenue, Toronto, ON M5T 2S8, Canada.
  • Ortín-Martínez A; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, 60 Leonard Avenue, Toronto, ON M5T 2S8, Canada.
  • Samuel Tsai EL; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Comanita L; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, 60 Leonard Avenue, Toronto, ON M5T 2S8, Canada.
  • Yan NE; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, 60 Leonard Avenue, Toronto, ON M5T 2S8, Canada.
  • Dolati P; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, 60 Leonard Avenue, Toronto, ON M5T 2S8, Canada.
  • Tachibana N; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, 60 Leonard Avenue, Toronto, ON M5T 2S8, Canada.
  • Liu ZC; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, 60 Leonard Avenue, Toronto, ON M5T 2S8, Canada.
  • Pearson JD; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada.
  • Chen D; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, 60 Leonard Avenue, Toronto, ON M5T 2S8, Canada.
  • Bremner R; Donald K. Johnson Eye Institute, Krembil Research Institute, University Health Network, 60 Leonard Avenue, Toronto, ON M5T 2S8, Canada.
  • Wallace VA; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5G 1X5, Canada.
iScience ; 24(8): 102905, 2021 Aug 20.
Article en En | MEDLINE | ID: mdl-34430805
The mouse eye is used to model central nervous system development, pathology, angiogenesis, tumorigenesis, and regenerative therapies. To facilitate the analysis of these processes, we developed an optimized tissue clearing and depigmentation protocol, termed InVision, that permits whole-eye fluorescent marker tissue imaging. We validated this method for the analysis of normal and degenerative retinal architecture, transgenic fluorescent reporter expression, immunostaining and three-dimensional volumetric (3DV) analysis of retinoblastoma and angiogenesis. We also used this method to characterize material transfer (MT), a recently described phenomenon of horizontal protein exchange that occurs between transplanted and recipient photoreceptors. 3D spatial distribution analysis of MT in transplanted retinas suggests that MT of cytoplasmic GFP between photoreceptors is mediated by short-range, proximity-dependent cellular interactions. The InVision protocol will allow investigators working across multiple cell biological disciplines to generate novel insights into the local cellular networks involved in cell biological processes in the eye.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: IScience Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: IScience Año: 2021 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos