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Smectite as a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice.
Breitrück, Anne; Weigel, Markus; Hofrichter, Jacqueline; Sempert, Kai; Kerkhoff, Claus; Mohebali, Nooshin; Mitzner, Steffen; Hain, Torsten; Kreikemeyer, Bernd.
Afiliación
  • Breitrück A; Extracorporeal Immunomodulation Unit (EXIM), Fraunhofer Institute for Cell Therapy and Immunology (IZI), 18057 Rostock, Germany.
  • Weigel M; Division of Nephrology, Department of Internal Medicine, University Medicine Rostock, 18057 Rostock, Germany.
  • Hofrichter J; Institute of Medical Microbiology, Justus Liebig University, 35392 Giessen, Germany.
  • Sempert K; Extracorporeal Immunomodulation Unit (EXIM), Fraunhofer Institute for Cell Therapy and Immunology (IZI), 18057 Rostock, Germany.
  • Kerkhoff C; Queensland Brain Institute, The University of Queensland, 4072 St Lucia, Brisbane 4000, Australia.
  • Mohebali N; Department of Human Sciences, School of Human Sciences, University of Osnabrück, 49076 Osnabrück, Germany.
  • Mitzner S; Institute of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany.
  • Hain T; Extracorporeal Immunomodulation Unit (EXIM), Fraunhofer Institute for Cell Therapy and Immunology (IZI), 18057 Rostock, Germany.
  • Kreikemeyer B; Division of Nephrology, Department of Internal Medicine, University Medicine Rostock, 18057 Rostock, Germany.
Int J Mol Sci ; 22(16)2021 Aug 13.
Article en En | MEDLINE | ID: mdl-34445403
ABSTRACT
Natural smectites have demonstrated efficacy in the treatment of diarrhea. The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis. Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11). Mice were sacrificed on day 21. Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction. Colon tissue was collected for histological damage scoring and quantification of tight junction protein expression. Stool samples were collected for microbiome analysis. Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS). No significant effects were obtained for the expression of TJ proteins (claudin-2 and claudin-3) after diosmectite treatment. Characterization of the microbial composition by 16S amplicon NGS showed that diosmectite treatment modified the DSS-associated dysbiosis. Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Sulfato de Dextran / Silicatos / Colitis / Microbiota Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Sulfato de Dextran / Silicatos / Colitis / Microbiota Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Alemania