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Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach.
Domingues, Luísa N; Bendele, Kylie G; Halos, Lénaïg; Moreno, Yovany; Epe, Christian; Figueiredo, Monica; Liebstein, Martin; Guerrero, Felix D.
Afiliación
  • Domingues LN; USDA-ARS Knipling-Bushland U. S. Livestock Insects Research Lab, 2700 Fredericksburg Road, Kerrville, TX, USA. luisa.domingues@tamu.edu.
  • Bendele KG; Texas A&M University, Department of Entomology, 2475 TAMU, College Station, TX, USA. luisa.domingues@tamu.edu.
  • Halos L; USDA-ARS Knipling-Bushland U. S. Livestock Insects Research Lab, 2700 Fredericksburg Road, Kerrville, TX, USA. kylie.bendele@usda.gov.
  • Moreno Y; Boehringer Ingelheim Animal Health, 29 Avenue Tony Garnier, 69007, Lyon, France.
  • Epe C; Bill and Melinda Gates Foundation, Seattle, WA, USA.
  • Figueiredo M; Boehringer Ingelheim Animal Health, Pharmaceutical Discovery and Research, 3239 Satellite Blvd. Bldg. 600, Duluth, GA, USA.
  • Liebstein M; Boehringer Ingelheim Animal Health, Pharmaceutical Discovery and Research, 3239 Satellite Blvd. Bldg. 600, Duluth, GA, USA.
  • Guerrero FD; Boehringer Ingelheim Animal Health, Pharmaceutical Discovery and Research, 3239 Satellite Blvd. Bldg. 600, Duluth, GA, USA.
Parasit Vectors ; 14(1): 442, 2021 Sep 03.
Article en En | MEDLINE | ID: mdl-34479607
ABSTRACT

BACKGROUND:

The horn fly, Haematobia irritans irritans, causes significant production losses to the cattle industry. Horn fly control relies on insecticides; however, alternative control methods such as vaccines are needed due to the fly's capacity to quickly develop resistance to insecticides, and the pressure for eco-friendly options.

METHODS:

We used a reverse vaccinology approach comprising three vaccine prediction and 11 annotation tools to evaluate and rank 79,542 translated open reading frames (ORFs) from the horn fly's transcriptome, and selected 10 transcript ORFs as vaccine candidates for expression in Pichia pastoris. The expression of the 10 selected transcripts and the proteins that they encoded were investigated in adult flies by reverse transcription polymerase chain reaction (RT-PCR) and mass spectrometry, respectively. Then, we evaluated the immunogenicity of a vaccine candidate in an immunization trial and the antigen's effects on horn fly mortality and fecundity in an in vitro feeding assay.

RESULTS:

Six of the ten vaccine candidate antigens were successfully expressed in P. pastoris. RT-PCR confirmed the expression of all six ORFs in adult fly RNA. One of the vaccine candidate antigens, BI-HS009, was expressed in sufficient quantity for immunogenicity and efficacy trials. The IgG titers of animals vaccinated with BI-HS009 plus adjuvant were significantly higher than those of animals vaccinated with buffer plus adjuvant only from days 42 to 112, with a peak on day 56. Progeny of horn flies feeding upon blood from animals vaccinated with BI-HS009 plus adjuvant collected on day 56 had 63% lower pupariation rate and 57% lower adult emergence than the control group (ANOVA F (1, 6) = 8.221, P = 0.028 and F (1, 6) = 8.299, P = 0.028, respectively).

CONCLUSIONS:

The reverse vaccinology approach streamlined the discovery process by prioritizing possible vaccine antigen candidates. Through a thoughtful process of selection and in vivo and in vitro evaluations, we were able to identify a promising antigen for an anti-horn fly vaccine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Muscidae / Vacunas / Enfermedades de los Bovinos / Inmunogenicidad Vacunal / Vacunología Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Parasit Vectors Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Muscidae / Vacunas / Enfermedades de los Bovinos / Inmunogenicidad Vacunal / Vacunología Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Parasit Vectors Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM