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Liquid-Liquid Phase Separation at the Plasma Membrane-Cytosol Interface: Common Players in Adhesion, Motility, and Synaptic Function.
Ramella, Martina; Ribolla, Lucrezia Maria; de Curtis, Ivan.
Afiliación
  • Ramella M; Vita-Salute San Raffaele University and San Raffaele Scientific Institute, Via Olgettina, 58, 20132 Milano, Italy. Electronic address: ramella.martina@hsr.it.
  • Ribolla LM; Vita-Salute San Raffaele University and San Raffaele Scientific Institute, Via Olgettina, 58, 20132 Milano, Italy. Electronic address: ribolla.lucreziamaria@hsr.it.
  • de Curtis I; Vita-Salute San Raffaele University and San Raffaele Scientific Institute, Via Olgettina, 58, 20132 Milano, Italy. Electronic address: decurtis.ivan@hsr.it.
J Mol Biol ; 434(1): 167228, 2022 01 15.
Article en En | MEDLINE | ID: mdl-34487789
ABSTRACT
Networks of scaffold proteins and enzymes assemble at the interface between the cytosol and specific sites of the plasma membrane, where these networks guide distinct cellular functions. Some of these plasma membrane-associated platforms (PMAPs) include shared core components that are able to establish specific protein-protein interactions, to produce distinct supramolecular assemblies regulating dynamic processes as diverse as cell adhesion and motility, or the formation and function of neuronal synapses. How cells organize such dynamic networks is still an open question. In this review we introduce molecular networks assembling at the edge of migrating cells, and at pre- and postsynaptic sites, which share molecular players that can drive the assembly of biomolecular condensates. Very recent experimental evidence has highlighted the emerging role of some of these multidomain/scaffold proteins belonging to the GIT, liprin-α and ELKS/ERC families as drivers of liquid-liquid phase separation (LLPS). The data point to an important role of LLPS (i) in the formation of PMAPs at the edge of migrating cells, where LLPS appears to be involved in promoting protrusion and the turnover of integrin-mediated adhesions, to allow forward cell translocation; (ii) in the assembly of the presynaptic active zone and of the postsynaptic density deputed to the release and reception of neurotransmitter signals, respectively. The recent results indicate that LLPS at cytosol-membrane interfaces is suitable not only for the regulation of active cellular processes, but also for the continuous spatial rearrangements of the molecular interactions involved in these dynamic processes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sinapsis / Proteínas / Membrana Celular / Citosol Límite: Animals / Humans Idioma: En Revista: J Mol Biol Año: 2022 Tipo del documento: Article Pais de publicación: HOLANDA / HOLLAND / NETHERLANDS / NL / PAISES BAJOS / THE NETHERLANDS

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sinapsis / Proteínas / Membrana Celular / Citosol Límite: Animals / Humans Idioma: En Revista: J Mol Biol Año: 2022 Tipo del documento: Article Pais de publicación: HOLANDA / HOLLAND / NETHERLANDS / NL / PAISES BAJOS / THE NETHERLANDS