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Exercise, but Not Metformin Prevents Loss of Muscle Function Due to Doxorubicin in Mice Using an In Situ Method.
Mackay, Amy D; Marchant, Erik D; Louw, Makensie; Thomson, David M; Hancock, Chad R.
Afiliación
  • Mackay AD; Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT 84602, USA.
  • Marchant ED; Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT 84602, USA.
  • Louw M; Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT 84602, USA.
  • Thomson DM; Cell Biology and Physiology, Brigham Young University, Provo, UT 84602, USA.
  • Hancock CR; Nutrition, Dietetics, and Food Science, Brigham Young University, Provo, UT 84602, USA.
Int J Mol Sci ; 22(17)2021 Aug 25.
Article en En | MEDLINE | ID: mdl-34502073
ABSTRACT
Though effective in treating various types of cancer, the chemotherapeutic doxorubicin (DOX) is associated with skeletal muscle wasting and fatigue. The purpose of this study was to assess muscle function in situ following DOX administration in mice. Furthermore, pre-treatments with exercise (EX) or metformin (MET) were used in an attempt to preserve muscle function following DOX. Mice were assigned to the following groups control, DOX, DOX + EX, or DOX + MET, and were given a single injection of DOX (15 mg/kg) or saline 3 days prior to sacrifice. Preceding the DOX injection, DOX + EX mice performed 60 min/day of running for 5 days, while DOX + MET mice received 5 daily oral doses of 500 mg/kg MET. Gastrocnemius-plantaris-soleus complex function was assessed in situ via direct stimulation of the sciatic nerve. DOX treatment increased time to half-relaxation following contractions, indicating impaired recovery (p < 0.05). Interestingly, EX prevented any increase in half-relaxation time, while MET did not. An impaired relaxation rate was associated with a reduction in SERCA1 protein content (p = 0.07) and AMPK phosphorylation (p < 0.05). There were no differences between groups in force production or mitochondrial respiration. These results suggest that EX, but not MET may be an effective strategy for the prevention of muscle fatigue following DOX administration in mice.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carrera / Músculo Esquelético / Fatiga Muscular / Metformina Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carrera / Músculo Esquelético / Fatiga Muscular / Metformina Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos