Development and application of a SARS-CoV-2 colorimetric biosensor based on the peroxidase-mimic activity of γ-Fe2O3 nanoparticles.
Mikrochim Acta
; 188(10): 335, 2021 Sep 09.
Article
en En
| MEDLINE
| ID: mdl-34505191
ABSTRACT
A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic γ Fe2O3 nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of γ Fe2O3 nanoparticles, the color change of H2O2 included 3,3',5,5'-tetramethylbenzidine was monitored at the wavelength of 654 nm when spike protein interacted with angiotensin-converting enzyme 2 receptor. This oxidation-reduction reaction was examined both spectroscopically and by using electrochemical techniques. The experimental parameters were optimized and the analytical characteristics investigated. The developed assay was applied to real SARS-CoV-2 samples, and very good results that were in accordance with the real time polymerase chain reaction were obtained.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colorimetría
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Glicoproteína de la Espiga del Coronavirus
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Nanopartículas Magnéticas de Óxido de Hierro
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Prueba de COVID-19
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SARS-CoV-2
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COVID-19
Límite:
Humans
Idioma:
En
Revista:
Mikrochim Acta
Año:
2021
Tipo del documento:
Article
País de afiliación:
Turquía