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The First Chemically-Synthesised, Highly Immunogenic Anti-SARS-CoV-2 Peptides in DNA Genotyped Aotus Monkeys for Human Use.
Patarroyo, Manuel E; Patarroyo, Manuel A; Alba, Martha P; Pabon, Laura; Rugeles, María T; Aguilar-Jimenez, Wbeimar; Florez, Lizdany; Bermudez, Adriana; Rout, Ashok K; Griesinger, Christian; Suarez, Carlos F; Aza-Conde, Jorge; Reyes, César; Avendaño, Catalina; Samacá, Jhoan; Camargo, Anny; Silva, Yolanda; Forero, Martha; Gonzalez, Edgardo.
Afiliación
  • Patarroyo ME; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Patarroyo MA; Universidad Santo Tomás, Bogotá, Colombia.
  • Alba MP; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Pabon L; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Rugeles MT; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Aguilar-Jimenez W; Grupo Inmunovirología, Universidad de Antioquia, Medellín, Colombia.
  • Florez L; Grupo Inmunovirología, Universidad de Antioquia, Medellín, Colombia.
  • Bermudez A; Grupo Inmunovirología, Universidad de Antioquia, Medellín, Colombia.
  • Rout AK; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Griesinger C; Department of NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Suarez CF; Department of NMR Based Structural Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
  • Aza-Conde J; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Reyes C; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Avendaño C; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Samacá J; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Camargo A; Universidad de Ciencias Aplicadas y Ambientales (U.D.C.A), Bogotá, Colombia.
  • Silva Y; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Forero M; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
  • Gonzalez E; Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá, Colombia.
Front Immunol ; 12: 724060, 2021.
Article en En | MEDLINE | ID: mdl-34539660
Thirty-five peptides selected from functionally-relevant SARS-CoV-2 spike (S), membrane (M), and envelope (E) proteins were suitably modified for immunising MHC class II (MHCII) DNA-genotyped Aotus monkeys and matched with HLA-DRß1* molecules for use in humans. This was aimed at producing the first minimal subunit-based, chemically-synthesised, immunogenic molecules (COLSARSPROT) covering several HLA alleles. They were predicted to cover 48.25% of the world's population for 6 weeks (short-term) and 33.65% for 15 weeks (long-lasting) as they induced very high immunofluorescent antibody (IFA) and ELISA titres against S, M and E parental native peptides, SARS-CoV-2 neutralising antibodies and host cell infection. The same immunological methods that led to identifying new peptides for inclusion in the COLSARSPROT mixture were used for antigenicity studies. Peptides were analysed with serum samples from patients suffering mild or severe SARS-CoV-2 infection, thereby increasing chemically-synthesised peptides' potential coverage for the world populations up to 62.9%. These peptides' 3D structural analysis (by 1H-NMR acquired at 600 to 900 MHz) suggested structural-functional immunological association. This first multi-protein, multi-epitope, minimal subunit-based, chemically-synthesised, highly immunogenic peptide mixture highlights such chemical synthesis methodology's potential for rapidly obtaining very pure, highly reproducible, stable, cheap, easily-modifiable peptides for inducing immune protection against COVID-19, covering a substantial percentage of the human population.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas de Subunidad / Glicoproteína de la Espiga del Coronavirus / Vacunas contra la COVID-19 / Proteínas M de Coronavirus / Proteínas de la Envoltura de Coronavirus / COVID-19 Límite: Animals / Humans Idioma: En Revista: Front Immunol Año: 2021 Tipo del documento: Article País de afiliación: Colombia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vacunas de Subunidad / Glicoproteína de la Espiga del Coronavirus / Vacunas contra la COVID-19 / Proteínas M de Coronavirus / Proteínas de la Envoltura de Coronavirus / COVID-19 Límite: Animals / Humans Idioma: En Revista: Front Immunol Año: 2021 Tipo del documento: Article País de afiliación: Colombia Pais de publicación: Suiza