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Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG.
Arimura, Yuki; Minato, Yusuke; Wada, Takayuki; Nakayama, Masaaki; Ryumon, Ayako; Hirata, Nao; Nakajima, Chie; Suzuki, Yasuhiko; Ato, Manabu; Kobayashi, Kazuo; Ohara, Naoko; Iida, Seiji; Ohara, Naoya.
Afiliación
  • Arimura Y; Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
  • Minato Y; Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Japan.
  • Wada T; Department of Microbiology, Graduate School of Human Life Science, Osaka City University, Osaka, Japan.
  • Nakayama M; Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
  • Ryumon A; Advanced Research Center for Oral and Craniofacial Sciences, Dental School, Okayama University, Okayama, Japan.
  • Hirata N; Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
  • Nakajima C; Department of Microbiology, Fujita Health University School of Medicine, Toyoake, Japan.
  • Suzuki Y; Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo, Japan.
  • Ato M; Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo, Japan.
  • Kobayashi K; Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
  • Ohara N; Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.
  • Iida S; Department of Operative Dentistry, Okayama University Hospital, Okayama University, Okayama, Japan.
  • Ohara N; Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Microbiol Immunol ; 66(1): 10-14, 2022 Jan.
Article en En | MEDLINE | ID: mdl-34546594
ABSTRACT
Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non-essentiality of thyX under a certain genetic background.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mycobacterium bovis / Mycobacterium tuberculosis Límite: Humans Idioma: En Revista: Microbiol Immunol Año: 2022 Tipo del documento: Article País de afiliación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mycobacterium bovis / Mycobacterium tuberculosis Límite: Humans Idioma: En Revista: Microbiol Immunol Año: 2022 Tipo del documento: Article País de afiliación: Japón