Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG.
Microbiol Immunol
; 66(1): 10-14, 2022 Jan.
Article
en En
| MEDLINE
| ID: mdl-34546594
ABSTRACT
Mycobacterium tuberculosis, the causative agent of tuberculosis, possess flavin-dependent thymidylate synthase, ThyX. Since thyX is absent in humans and was shown to be essential for M. tuberculosis normal growth, ThyX is thought to be an attractive novel TB drug target. This study assessed thyX essentiality in Mycobacterium bovis BCG strains using CRISPR interference based gene silencing and found that thyX is not essential in an M. bovis BCG Tokyo derivative strain. A thyX deletion mutant strain was successfully constructed from that strain, which reinforces the non-essentiality of thyX under a certain genetic background.
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Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Mycobacterium bovis
/
Mycobacterium tuberculosis
Límite:
Humans
Idioma:
En
Revista:
Microbiol Immunol
Año:
2022
Tipo del documento:
Article
País de afiliación:
Japón