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A new carbohydrate-active oligosaccharide dehydratase is involved in the degradation of ulvan.
Bäumgen, Marcus; Dutschei, Theresa; Bartosik, Daniel; Suster, Christoph; Reisky, Lukas; Gerlach, Nadine; Stanetty, Christian; Mihovilovic, Marko D; Schweder, Thomas; Hehemann, Jan-Hendrik; Bornscheuer, Uwe T.
Afiliación
  • Bäumgen M; Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University Greifswald, Greifswald, Germany.
  • Dutschei T; Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University Greifswald, Greifswald, Germany.
  • Bartosik D; Department of Pharmaceutical Biotechnology, Institute of Pharmacy, University Greifswald, Greifswald, Germany.
  • Suster C; Institute of Applied Synthetic Chemistry, TU Wien, Vienna, Austria.
  • Reisky L; Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University Greifswald, Greifswald, Germany.
  • Gerlach N; Max Planck-Institute for Marine Microbiology, Bremen, Germany; Center for Marine Environmental Sciences (MARUM), University of Bremen, Bremen, Germany.
  • Stanetty C; Institute of Applied Synthetic Chemistry, TU Wien, Vienna, Austria.
  • Mihovilovic MD; Institute of Applied Synthetic Chemistry, TU Wien, Vienna, Austria.
  • Schweder T; Department of Pharmaceutical Biotechnology, Institute of Pharmacy, University Greifswald, Greifswald, Germany.
  • Hehemann JH; Max Planck-Institute for Marine Microbiology, Bremen, Germany; Center for Marine Environmental Sciences (MARUM), University of Bremen, Bremen, Germany.
  • Bornscheuer UT; Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University Greifswald, Greifswald, Germany. Electronic address: uwe.bornscheuer@uni-greifswald.de.
J Biol Chem ; 297(4): 101210, 2021 10.
Article en En | MEDLINE | ID: mdl-34547290
ABSTRACT
Marine algae catalyze half of all global photosynthetic production of carbohydrates. Owing to their fast growth rates, Ulva spp. rapidly produce substantial amounts of carbohydrate-rich biomass and represent an emerging renewable energy and carbon resource. Their major cell wall polysaccharide is the anionic carbohydrate ulvan. Here, we describe a new enzymatic degradation pathway of the marine bacterium Formosa agariphila for ulvan oligosaccharides involving unsaturated uronic acid at the nonreducing end linked to rhamnose-3-sulfate and glucuronic or iduronic acid (Δ-Rha3S-GlcA/IdoA-Rha3S). Notably, we discovered a new dehydratase (P29_PDnc) acting on the nonreducing end of ulvan oligosaccharides, i.e., GlcA/IdoA-Rha3S, forming the aforementioned unsaturated uronic acid residue. This residue represents the substrate for GH105 glycoside hydrolases, which complements the enzymatic degradation pathway including one ulvan lyase, one multimodular sulfatase, three glycoside hydrolases, and the dehydratase P29_PDnc, the latter being described for the first time. Our research thus shows that the oligosaccharide dehydratase is involved in the degradation of carboxylated polysaccharides into monosaccharides.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Proteínas Bacterianas / Deshidrogenasas de Carbohidratos / Flavobacteriaceae / Organismos Acuáticos Idioma: En Revista: J Biol Chem Año: 2021 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Proteínas Bacterianas / Deshidrogenasas de Carbohidratos / Flavobacteriaceae / Organismos Acuáticos Idioma: En Revista: J Biol Chem Año: 2021 Tipo del documento: Article País de afiliación: Alemania