Interaction of amino acids with nanoplastic traces and their effect on Staphylococcus aureus.

ABSTRACT

In this study, we reported the interaction between plastic traces and vital amino acids (L-homocysteine, L-valine, and L-lysine) in an aqueous system and characterized this interaction by Fourier transform infrared spectroscopy and Scanning electron microscopy with energy-dispersive X-ray spectroscopy studies. Bacterial activity and biofilm formation and their characteristics of non-treated and amino acid-treated plastic traces was tested against the Staphylococcus aureus bacterial pathogen. The surface results showed that the carbonyl groups and oxygen to carbon ratios were increased, and the attachment of nitrogen- and sulfur-related substances on the plastic surface occurred by the homocysteine over time. Plastic traces showed particle surface deformation using the main functional groups (e. g. alkyne-alkene, vinyl, secondary alcohols, alkane-methylene) with the increasing lysine treatment; however, decreased oxygen to carbon ratio showed particle anti-aging. The most common functional groups were primarily deformed with the longer exposure to valine. The bacterial activity results showed that the Staphylococcus aureus activities were not primarily changed by the amino acid treatment compared to the non-treated plastic traces. However, amino acid treated plastic traces induced the biofilm formation and its characteristic due to surface deformation of functional groups and alteration of new substances on plastic traces.