Immunization of chickens with Salmonella gallinarium ghosts expressing Salmonella Enteritidis NFliC-FimAC and CD40LC fusion antigen enhances cell-mediated immune responses and protects against wild-type challenges with both species.
Dev Comp Immunol
; 126: 104265, 2022 01.
Article
en En
| MEDLINE
| ID: mdl-34555416
ABSTRACT
This study describes the construction and immunological characterization of a novel Salmonella gallinarium ghost vaccine to protect against S. gallinarium (SG) and S. Enteritidis (SE) serotypes. The SG ghost was designed to express N-terminus FliC (D0-D1 domain) and FimA retrieved from the SE genome, and the receptor-binding domain (RBD) of CD40L from the chicken as a single fusion construct. The construct was built in pJHL184, a phage lysis gene E-mediated ghost plasmid and the expression was confirmed by western blot resulting in an 85-kDa band. Chicken immunization was conducted by intramuscular route with SG ghost FliC-FimA-CD40L, vector control, or PBS alone in a prime-boost schedule. Antibody responses, cell-mediated immune responses (CMI), and cytokine induction was assessed in chicken demonstrating significantly high levels of IgY, CMI, cytokine responses in ghost immunized group delivering partial protection against SG wild type challenge and near complete protection against SE challenge wild type challenge.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
/
Salmonelosis Animal
Límite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Año:
2022
Tipo del documento:
Article
País de afiliación:
Corea del Sur