Simplification of two-dimensional gel patterns of HLA class II antigens.
Tissue Antigens
; 28(2): 72-83, 1986 Aug.
Article
en En
| MEDLINE
| ID: mdl-3462963
ABSTRACT
Two-dimensional gel electrophoresis of material immunoprecipitated from radiolabeled cells by anti-HLA class II monoclonal antibodies has been a useful technique but the patterns observed have varied considerably between laboratories. In this study we investigated the parameters that vary between different laboratories. We found that heterogeneity due to post synthetic modification of beta and alpha chains of DR and DQ can be avoided by labeling cells for 30 min with 35S-methionine. A mixture of ampholytes pH range 3.5-105-7 of 14 run for 3000 V/h gave the best resolution of beta and alpha chains. With this technique we could clearly reveal differences in the DR beta-2 chains of those DR2 haplotypes, Dw2, Dw12 and LD'tb24. The Dr beta 1 position varied between different DR types.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Antígenos de Histocompatibilidad Clase II
/
Electroforesis en Gel de Poliacrilamida
Límite:
Humans
Idioma:
En
Revista:
Tissue Antigens
Año:
1986
Tipo del documento:
Article