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Eugenol and its liposome-based nano carrier reduce anxiety by inhibiting glyoxylase-1 expression in mice.
Siyal, F J; Siddiqui, R A; Memon, Z; Aslam, Z; Nisar, U; Imad, R; Shah, M R.
Afiliación
  • Siyal FJ; Ziauddin University, Faculty of Pharmacy, Department of Pharmacology, Karachi, Pakistan.
  • Siddiqui RA; Shaheed Mohtarma Benazir Bhutto Medical University, Institute of Pharmacy, Larkana, Pakistan.
  • Memon Z; Ziauddin University, Department of Research, Karachi, Pakistan.
  • Aslam Z; Ziauddin University, Ziauddin Medical College, Department of Pharmacology, Karachi, Pakistan.
  • Nisar U; University of Karachi, International Center for Chemical and Biological Sciences, HEJ Research Institute of Chemistry, Karachi, Pakistan.
  • Imad R; Ziauddin University, Faculty of Pharmacy, Department of Pharmacology, Karachi, Pakistan.
  • Shah MR; Ziauddin University, Department of Research, Karachi, Pakistan.
Braz J Biol ; 83: e251219, 2021.
Article en En | MEDLINE | ID: mdl-34669914
The most common form of psycho-social dysfunction is anxiety with depression being related closely without any age bar. They are present with combined state of sadness, confusion, stress, fear etc. Glyoxalase system contains enzyme named glyoxalase 1 (GLO1).It is a metabolic pathway which detoxifies alpha-oxo-aldehydes, particularly methylglyoxal (MG). Methylglyoxal is mainly made by the breakdown of the glycolytic intermediates, glyceraldehyde-3-phosphates and dihydroxyacetone phosphate. Glyoxylase-1 expression is also related with anxiety behavior. A casual role or GLO-1 in anxiety behavior by using viral vectors for over expression in the anterior cingulate cortex was found and it was found that local GLO-1 over expression increased anxiety behavior. The present study deals with the molecular mechanism of protective activity of eugenol against anxiolytic disorder. A pre-clinical animal study was performed on 42 BALB/c mice. Animals were given stress through conventional restrain model. The mRNA expression of GLO-1 was analyzed by real time RT-PCR. Moreover, the GLO-1 protein expression was also examined by immunohistochemistry in whole brain and mean density was calculated. The mRNA and protein expressions were found to be increased in animals given anxiety as compared to the normal control. Whereas, the expressions were decreased in the animals treated with eugenol and its liposome-based nanocarriers in a dose dependent manner. However, the results were better in animals treated with nanocarriers as compared to the compound alone. It is concluded that the eugenol and its liposome-based nanocarriers exert anxiolytic activity by down-regulating GLO-1 protein expression in mice.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Eugenol / Lactoilglutatión Liasa Límite: Animals Idioma: En Revista: Braz J Biol Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Brasil

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Eugenol / Lactoilglutatión Liasa Límite: Animals Idioma: En Revista: Braz J Biol Asunto de la revista: BIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Brasil