A Cold-Shock Protein from the South Pole-Dwelling Soil Bacterium Arthrobacter sp. Confers Cold Tolerance to Rice.
Genes (Basel)
; 12(10)2021 10 09.
Article
en En
| MEDLINE
| ID: mdl-34680989
ABSTRACT
Low temperature is a critical environmental factor restricting the physiology of organisms across kingdoms. In prokaryotes, cold shock induces the expression of various genes and proteins involved in cellular processes. Here, a cold-shock protein (ArCspA) from the South Pole-dwelling soil bacterium Arthrobacter sp. A2-5 was introduced into rice, a monocot model plant species. Four-week-old 35SArCspA transgenic rice plants grown in a cold chamber at 4 °C survived for 6 days. Cold stress significantly decreased the chlorophyll content in WT plants after 4 days compared with that in 35SArCspA transgenic plants. RNA-seq analysis was performed on WT and 35SArCspA transgenic rice with/without cold stress. GO terms such as "response to stress (GO0006950)", "response to cold (GO0009409)", and "response to heat (GO0009408)" were significantly enriched among the upregulated genes in the 35SArCspA transgenic rice under normal conditions, even without cold-stress treatment. The expression of five cold stress-related genes, Rab16B (Os11g0454200), Rab21 (Os11g0454300), LEA22 (Os01g0702500), ABI5 (Os01 g0859300), and MAPK5 (Os03g0285800), was significantly upregulated in the transgenic rice compared with the WT rice. These results indicate that the ArCspA gene might be involved in the induction of cold-responsive genes and provide cold tolerance.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arthrobacter
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Oryza
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Microbiología del Suelo
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Adaptación Fisiológica
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Frío
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Proteínas y Péptidos de Choque por Frío
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Genes (Basel)
Año:
2021
Tipo del documento:
Article