An α-D-galactan and a ß-D-glucan from the mushroom Amanita muscaria: Structural characterization and antitumor activity against melanoma.
Carbohydr Polym
; 274: 118647, 2021 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-34702466
ABSTRACT
Polysaccharides α-D-galactan (GAL-Am) and ß-D-glucan (GLC-Am) were obtained from Amanita muscaria fruiting bodies. They were purified using different methodologies, such as Fehling precipitation (for both fractions), freeze-thawing process and ultrafiltration (for GLC-Am). Results showed that the GAL-Am has (1 â 6)-linked Galp main chain branched at O-2 by terminal Galp units and has not been previously reported. Besides, GLC-Am has (1 â 3)-linked Glcp in the main chain, substituted at O-6 by (1 â 6)-linked ß-Glcp units. Both are water-soluble, with 9.0 × 103 g/moL and 1.3 × 105 g/moL, respectively. GAL-Am and GLC-Am presented a selective proliferation reduction against B16-F10 melanoma cell line, not affecting non tumoral BALB/3T3 fibroblast cell line. Furthermore, both fractions reduced clonogenic capacity of melanoma cell line over an extended period of time. These results were obtained without modulations in B16-F10 cell adhesion, reinforcing the biological activities towards cell proliferation impairment and eliciting these polysaccharides as promising compounds to further exploration of their antimelanoma properties.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Melanoma Experimental
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Amanita
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Galactanos
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Glucanos
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Antineoplásicos
Límite:
Animals
Idioma:
En
Revista:
Carbohydr Polym
Año:
2021
Tipo del documento:
Article
País de afiliación:
Brasil