Toxic Effect of Streptozotocin on Cultured Mouse Hippocampal Neurons.
Bull Exp Biol Med
; 171(6): 732-735, 2021 Oct.
Article
en En
| MEDLINE
| ID: mdl-34705175
ABSTRACT
In primary dissociated hippocampal cell cultures from 18-day-old mouse embryos, streptozotocin in concentrations of 2-5 mM produced a dose-dependent cytotoxic effect on day 3 in vitro, whereas on day 11 of culturing, the neurons were resistant to streptozotocin. The neurons in the 3-day cultures were functionally immature, which was seen from their weak spontaneous bioelectric activity in the form of rare single action potentials; by day 11 of culturing, the neurons reached a high level of differentiation and their functional properties acquired a character of network burst activity. Thus, streptozotocin had the most pronounced cytotoxic effect on immature hippocampal neurons in vitro.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Estreptozocina
/
Antibióticos Antineoplásicos
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Bull Exp Biol Med
Año:
2021
Tipo del documento:
Article
País de afiliación:
Rusia