Your browser doesn't support javascript.
loading
Performance characteristics of chimerism testing by next generation sequencing.
Cusick, Matthew F; Clark, Lauren; Tu, Thuydung; Goforth, John; Zhang, Xiaohai; LaRue, Bobby; Gutierrez, Ryan; Jindra, Peter T.
Afiliación
  • Cusick MF; Department of Pathology, University of Michigan Medicine, 2800 Plymouth Rd., Building 36, Ann Arbor, MI 48109, USA. Electronic address: mfcusick@med.umich.edu.
  • Clark L; Department of Surgery, Baylor College of Medicine, One Baylor Plaza, MS:BCM 504, Houston, TX 77030, USA.
  • Tu T; Department of Surgery, Baylor College of Medicine, One Baylor Plaza, MS:BCM 504, Houston, TX 77030, USA.
  • Goforth J; Department of Surgery, Baylor College of Medicine, One Baylor Plaza, MS:BCM 504, Houston, TX 77030, USA.
  • Zhang X; HLA and Immunogenetics, Laboratory, Cedars-Sinai Medical Center, Lab-SSB 197, 8723 Alden D, Los Angeles, CA 90048, USA.
  • LaRue B; Department of Forensic Science, 1003 Bowers Blvd, Sam Houston, State University Huntsville, TX 77340, USA.
  • Gutierrez R; Department of Forensic Science, 1003 Bowers Blvd, Sam Houston, State University Huntsville, TX 77340, USA.
  • Jindra PT; Department of Surgery, Baylor College of Medicine, One Baylor Plaza, MS:BCM 504, Houston, TX 77030, USA. Electronic address: Peter.Jindra@bcm.edu.
Hum Immunol ; 83(1): 61-69, 2022 Jan.
Article en En | MEDLINE | ID: mdl-34728094
Chimerism testing provides informative clinical data regarding the status of a biological sample mixture. For years, this testing was achieved by measuring the peaks of informative short tandem repeat (STR) loci using capillary electrophoresis (CE). With the advent of next generation sequencing (NGS) technology, the quantification of the percentage of donor/recipient mixtures is more easily done using sequence reads in large batches of samples run on a single flow cell. In this study, we present data on using a FORENSIC NGS chimerism platform to accurately measure the percentage of donor/recipient mixtures. We were able to detect chimerism to a limit threshold of 1% using both STR and single nucleotide polymorphism (SNP) informative loci. Importantly, a significant correlation was observed between NGS and CE chimerism methods when compared at donor detection ranges from 1% to 10%. Furthermore, 100% accuracy was achieved through proficiency testing over six surveys. Its usefulness was expanded beyond this to help identify suitable donors for solid organ transplant patients using ancestry SNP profiles. In summary, the NGS method provides a sensitive and reliable alternative to traditional CE for chimerism testing of clinical samples.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quimerismo / Secuenciación de Nucleótidos de Alto Rendimiento Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Hum Immunol Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quimerismo / Secuenciación de Nucleótidos de Alto Rendimiento Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Hum Immunol Año: 2022 Tipo del documento: Article Pais de publicación: Estados Unidos