Cross-Linking Mass Spectrometry for Investigating Protein Conformations and Protein-Protein InteractionsâA Method for All Seasons.
Chem Rev
; 122(8): 7500-7531, 2022 04 27.
Article
en En
| MEDLINE
| ID: mdl-34797068
Mass spectrometry (MS) has become one of the key technologies of structural biology. In this review, the contributions of chemical cross-linking combined with mass spectrometry (XL-MS) for studying three-dimensional structures of proteins and for investigating protein-protein interactions are outlined. We summarize the most important cross-linking reagents, software tools, and XL-MS workflows and highlight prominent examples for characterizing proteins, their assemblies, and interaction networks in vitro and in vivo. Computational modeling plays a crucial role in deriving 3D-structural information from XL-MS data. Integrating XL-MS with other techniques of structural biology, such as cryo-electron microscopy, has been successful in addressing biological questions that to date could not be answered. XL-MS is therefore expected to play an increasingly important role in structural biology in the future.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas
Idioma:
En
Revista:
Chem Rev
Año:
2022
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Estados Unidos