GLO 1 and PKCλ Regulate ALDH1-positive Breast Cancer Stem Cell Survival.
Anticancer Res
; 41(12): 5959-5971, 2021 Dec.
Article
en En
| MEDLINE
| ID: mdl-34848450
ABSTRACT
BACKGROUND/AIM:
We examined the inhibitory effects of both glyoxalase 1 (GLO 1) and protein kinase C (PKC)λ in aldehyde dehydrogenase 1 (ALDH1)-positive breast cancer stem cells (CSCs). MATERIALS ANDMETHODS:
Breast cancer genomics datasets (TCGA, n=593; METABRIC, n=1904) were downloaded and statistically analyzed. The effects of GLO 1 and PKCλ on trypan blue staining and tumor-sphere formation by ALDH1high cells derived from triple negative breast cancer (TNBC) and basal-like breast cancer were examined.RESULTS:
GLO 1high, PKCλhigh, and ALDH1A3high tumors were enriched in stage I/II/III/IV samples, associated with the HER2 and TNBC subtypes according to receptor status, and associated with the HER2-enriched and basal-like subtypes according to PAM50. Inhibition of either GLO 1 (TLSC702) or PKCλ (ANF) suppressed tumor-sphere formation and enhanced death in ALDH1high cells. TLSC702 also effectively inhibited tumor-sphere formation and induced death in PKCλ knockout ALDH1high cells.CONCLUSION:
GLO 1 and PKCλ are important for the survival of ALDH1-positive breast CSCs, and may represent potential therapeutic targets for the treatment of ALDH1-positive breast CSCs.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre Neoplásicas
/
Proteína Quinasa C
/
Neoplasias de la Mama
/
Familia de Aldehído Deshidrogenasa 1
/
Isoenzimas
/
Lactoilglutatión Liasa
Tipo de estudio:
Etiology_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
Anticancer Res
Año:
2021
Tipo del documento:
Article
País de afiliación:
Japón