The versatile electric condition in mouse embryos for genome editing using a three-step square-wave pulse electroporator.

Nakano, Kenta; Shimizu, Yukiko; Arai, Tetsuya; Kaneko, Taketo; Okamura, Tadashi

Nakano, Kenta; Shimizu, Yukiko; Arai, Tetsuya; Kaneko, Taketo; Okamura, Tadashi.

Afiliación

Nakano K; Department of Laboratory Animal Medicine, Research Institute, National Center for Global Health and Medicine (NCGM), 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
Shimizu Y; Department of Laboratory Animal Medicine, Research Institute, National Center for Global Health and Medicine (NCGM), 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
Arai T; Department of Laboratory Animal Medicine, Research Institute, National Center for Global Health and Medicine (NCGM), 1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655, Japan.
Kaneko T; Department of Chemistry and Biological Sciences, Faculty of Science and Engineering, Iwate University, 4-3-5 Ueda, Morioka, Iwate 020-8551, Japan.
Okamura T; Division of Fundamental and Applied Sciences, Graduate School of Science and Engineering, Iwate University, 4-3-5 Ueda, Morioka, Iwate 020-8551, Japan.

Exp Anim ; 71(2): 214-223, 2022 May 20.

Article en En | MEDLINE | ID: mdl-34880157

Asunto(s)

Sistemas CRISPR-Cas; Edición Génica; Animales; Edición Génica/métodos; Ratones; Ratones Endogámicos C3H; Ratones Endogámicos C57BL; Ratones Noqueados

Palabras clave

CRISPR/Cas9; electroporation; frozen-thawed embryos; genome editing

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistemas CRISPR-Cas / Edición Génica Límite: Animals Idioma: En Revista: Exp Anim Asunto de la revista: MEDICINA VETERINARIA Año: 2022 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón

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