[Effect of PRRX1 on autophagy of salivary adenoid cystic carcinoma cells].
Shanghai Kou Qiang Yi Xue
; 30(5): 477-480, 2021 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-34888598
ABSTRACT
PURPOSE:
To investigate the effect of paired related homeobox1 (PRRX1) on autophagy of salivary adenoidcystic carcinoma (SACC) cells.METHODS:
PRRX1-overexpressed lentiviral vectors and their negative control lentiviral vectors were used to transfect SACC cells. The transfection effect was detected by Western blot. Autophagosome was observed under transmission electron microscopy(TEM). The level of autophagy markers (LC3-II/Beclin1) was detected by immunofluorescence and Western blot. Statistical analysis was performed with SPSS 17.0 software package.RESULTS:
PRRX1 protein level increased, autophagosome number decreased, and autophagy marker level decreased in the PRRX1 overexpressed group, compared to the control (P<0.05).CONCLUSIONS:
PRRX1 inhibits the autophagy of SACC cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de las Glándulas Salivales
/
Carcinoma Adenoide Quístico
Límite:
Humans
Idioma:
Zh
Revista:
Shanghai Kou Qiang Yi Xue
Asunto de la revista:
ODONTOLOGIA
Año:
2021
Tipo del documento:
Article