Comparison of HPLC and NMR for quantification of the main volatile fatty acids in rumen digesta.
Sci Rep
; 11(1): 24337, 2021 12 21.
Article
en En
| MEDLINE
| ID: mdl-34934079
ABSTRACT
Accurate quantification of volatile fatty acid (VFA) concentrations in rumen fluid are essential for research on rumen metabolism. The study comprehensively investigated the pros and cons of High-performance liquid chromatography (HPLC) and 1H Nuclear magnetic resonance (1H-NMR) analysis methods for rumen VFAs quantification. We also investigated the performance of several commonly used data pre-treatments for the two sets of data using correlation analysis, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). The molar proportion and reliability analysis demonstrated that the two approaches produce highly consistent VFA concentrations. In the pre-processing of NMR spectra, line broadening and shim correction may reduce estimated concentrations of metabolites. We observed differences in results using multiplet of different protons from one compound and identified "handle signals" that provided the most consistent concentrations. Different data pre-treatment strategies tested with both HPLC and NMR significantly affected the results of downstream data analysis. "Normalized by sum" pre-treatment can eliminate a large number of positive correlations between NMR-based VFA. A "Combine" strategy should be the first choice when calculating the correlation between metabolites or between samples. The PCA and PLS-DA suggest that except for "Normalize by sum", pre-treatments should be used with caution.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rumen
/
Espectroscopía de Resonancia Magnética
/
Cromatografía Líquida de Alta Presión
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Dieta
/
Ácidos Grasos Volátiles
Límite:
Animals
Idioma:
En
Revista:
Sci Rep
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido