Phytochemical Composition and Anti-Aging Activity of Butanol Extract of Hedyotis diffusa in Caenorhabditis elegans.
Chem Biodivers
; 19(2): e202100685, 2022 Feb.
Article
en En
| MEDLINE
| ID: mdl-34935259
ABSTRACT
Hedyotis diffusa Willd. (H.â
diffusa), a kind of traditional Chinese medicine, has been evaluated to potential display antioxidant and anti-aging effects inâ
vitro experiments. In this work, we investigated the effects on lifespan and stress resistance of the butanol extract from H.â
diffusa (NHD) inâ
vivo using a Caenorhabditis elegans (C.â
elegans) model. The phytochemicals of NHD were identified by UPLC-ESI-qTOF-MS/MS method. NHD-treated wild-type N2 worms showed an increase in survival time under both normal and stress conditions. Meanwhile, NHD promoted the healthspan of nematodes by stimulating growth and development, reducing the deposition of age pigment, increasing the activities of superoxide dismutase (SOD) and glutathione peroxidase dismutase (GSH-Px), and decreasing the level of ROS without impairing fertility. Moreover, the upregulating of the expression of daf-16, gst-4, sod-3, hsp12.6 genes and the downregulating of the expression of daf-2 were involved in the NHD-mediated lifespan extension. Finally, the increasing of the expression of GST-4GFP in CL2166 transgenic nematodes and the life-span-extending activity of NHD was completely abolished in daf-2 and daf-16 mutants further revealed that the potential roles for these genes in NHD-induced longevity in C.â
elegans. Collectively, our findings suggest that NHD may have an active effect in healthy aging and age-related diseases.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Caenorhabditis elegans
/
Hedyotis
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Chem Biodivers
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2022
Tipo del documento:
Article
País de afiliación:
China