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The Dynamics of Circulating Heparin-Binding Protein: Implications for Its Use as a Biomarker.
Fisher, Jane; Kahn, Fredrik; Wiebe, Elena; Gustafsson, Pontus; Kander, Thomas; Mellhammar, Lisa; Bentzer, Peter; Linder, Adam.
Afiliación
  • Fisher J; Division of Infection Medicine, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
  • Kahn F; Division of Infection Medicine, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
  • Wiebe E; Division of Infection Medicine, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
  • Gustafsson P; Infection Biochemistry & Institute for Biochemistry, University of Veterinary Medicine Hanover, Hanover, Germany.
  • Kander T; Division of Infection Medicine, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
  • Mellhammar L; Department of Intensive and Perioperative Care, Skåne University Hospital, Lund, Sweden.
  • Bentzer P; Division of Anesthesiology and Intensive Care, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
  • Linder A; Division of Infection Medicine, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
J Innate Immun ; 14(5): 447-460, 2022.
Article en En | MEDLINE | ID: mdl-34965528
Heparin-binding protein (HBP) is a promising biomarker for the development and severity of sepsis. To guide its use, it is important to understand the factors that could lead to false-positive or negative results, such as inappropriate release and inadequate clearance of HBP. HBP is presumably released only by neutrophils, and the organs responsible for its elimination are unknown. In this study, we aimed to determine whether non-neutrophil cells can be a source of circulating HBP and which organs are responsible for its removal. We found that in two cohorts of neutropenic patients, 12% and 19% of patients in each cohort, respectively, had detectable plasma HBP levels. In vitro, three leukemia-derived monocytic cell lines and healthy CD14+ monocytes constitutively released detectable levels of HBP. When HBP was injected intravenously in rats, we found that plasma levels of HBP decreased rapidly, with a distribution half-life below 10 min and an elimination half-life of 1-2 h. We measured HBP levels in the liver, spleen, kidneys, lungs, and urine using both ELISA and immunofluorescence quantitation, and found that the majority of HBP was present in the liver, and a small amount was present in the spleen. Immunofluorescence imaging indicated that HBP is associated mainly with hepatocytes in the liver and monocytes/macrophages in the spleen. The impact of hematologic malignancies and liver diseases on plasma HBP levels should be explored further in clinical studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Sanguíneas / Sepsis Límite: Animals / Humans Idioma: En Revista: J Innate Immun Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Sanguíneas / Sepsis Límite: Animals / Humans Idioma: En Revista: J Innate Immun Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Suiza