Cell components, interaction types and functional groups involved in the in vitro binding of bisphenol A by Lactiplantibacillus plantarum RS20D and DL7X.

ABSTRACT

AIMS: The current study aimed to evaluate the capacity of two Lactiplantibacillus plantarum strains to remove Bisphenol A (BPA) and to determine the preliminary removal mechanisms underlying this process. METHODS AND RESULTS: The BPA removal capacity of L. plantarum RS20D and DL7X was assessed by HPLC analysis. The effect of various treatments (physical, chemical and enzymatic) on two strains were studied to understand which interaction types worked. The different cellular components of them were also subjected to binding assays. Additionally, Fourier-transform infrared spectroscopy (FTIR) was performed to identify the functional groups related to the BPA-binding process. Results show that various treatments enhanced the binding capacity of two strains, the effect of sodium dodecyl sulphate was the most outstanding (p < 0.05). Hydrogen bonding and hydrophobic interactions likely occurred. Peptidoglycans showed the highest binding capability, protoplasts and teichoic acids might also exert a binding effect. -OH, C=O, -CH, -NH, C-N, C-O and P=O participated in BPA binding by the two L. plantarum lines. CONCLUSIONS: Peptidoglycans, protoplasts and teichoic acid played a vital role in the binding of BPA. SIGNIFICANCE AND IMPACT OF THE STUDY Our results provided a theoretical foundation for developing effective dietary strategies with foodborne L. plantarum to remove food contaminants.