A new near-infrared fluorescent probe for sensing extreme acidity and bioimaging in lysosome.
Methods Appl Fluoresc
; 10(2)2022 Feb 10.
Article
en En
| MEDLINE
| ID: mdl-35073535
ABSTRACT
Since the intracellular pH plays an important role in the physiological and pathological processes, however, the probes that can be used for monitoring pH fluctuation under extreme acidic conditions are currently rare, so it is necessary to construct fluorescent probes for sensing pH less than 4. In this work, we developed a new near-infrared (NIR) fluorescent probeCy-SNNfor sensing pH fluctuation under extremely acidic conditions. For the preparation of this probe, benzothiozolium moiety was chosen as lysosomal targeting unit and NIR fluorophore, and barbituric acid moiety was fused in the polymethine chain of probe to introduce protonation center. Surprisingly, on the basis of the balance of quaternary ammonium salts and free amines, the pkavalue ofCy-SNNwas calculated as low as 2.96, implying thatCy-SNNcan be used in acidic conditions with pH < 4. Moreover,Cy-SNNexhibited highly selective response to H+over diverse analytes in real-time with dependable reversibility. Importantly,Cy-SNNcan be used to specifically target lysosome, providing potential tools for monitoring the function of lysosome in autophagy process.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colorantes Fluorescentes
/
Lisosomas
Idioma:
En
Revista:
Methods Appl Fluoresc
Año:
2022
Tipo del documento:
Article