Introduction of a Hexalysine (6 K) Tag Can Protect from N-Terminal Cleavage and Increase Yield of Recombinant Proteins Expressed in the Periplasm of E. coli.

Paraskevopoulou, Vasiliki; Alissa, Mohammed; Hage, Naim; Falcone, Franco H

Introduction of a Hexalysine (6 K) Tag Can Protect from N-Terminal Cleavage and Increase Yield of Recombinant Proteins Expressed in the Periplasm of E. coli.

Paraskevopoulou, Vasiliki; Alissa, Mohammed; Hage, Naim; Falcone, Franco H.

Afiliación

Paraskevopoulou V; Molecular Therapeutics and Formulation Division, School of Pharmacy, University of Nottingham, Nottingham, UK.
Alissa M; New Modalities and Parenteral Development, Pharmaceutical Technology & Development, Operations, AstraZeneca, Macclesfield, UK.
Hage N; Molecular Therapeutics and Formulation Division, School of Pharmacy, University of Nottingham, Nottingham, UK.
Falcone FH; Molecular Therapeutics and Formulation Division, School of Pharmacy, University of Nottingham, Nottingham, UK.

Methods Mol Biol ; 2406: 155-167, 2022.

Article en En | MEDLINE | ID: mdl-35089556

Asunto(s)

Proteínas de Escherichia coli; Periplasma; Escherichia coli/genética; Escherichia coli/metabolismo; Proteínas de Escherichia coli/metabolismo; Periplasma/metabolismo; Pliegue de Proteína; Proteínas Recombinantes/metabolismo

Palabras clave

Disulfide bond; Hexalysine tag; PelB leader; Periplasm; Yield

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periplasma / Proteínas de Escherichia coli Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos

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