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A self-immolative linker that releases thiols detects penicillin amidase and nitroreductase with high sensitivity via absorption spectroscopy.
Espinoza, Eli M; Røise, Joachim J; He, Maomao; Li, I-Che; Agatep, Alvin K; Udenyi, Patrick; Han, Hesong; Jackson, Nicole; Kerr, D Lucas; Chen, Dake; Stentzel, Michael R; Ruan, Emily; Riley, Lee; Murthy, Niren.
Afiliación
  • Espinoza EM; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA. nmurthy@berkeley.edu.
  • Røise JJ; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA. nmurthy@berkeley.edu.
  • He M; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Li IC; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA. nmurthy@berkeley.edu.
  • Agatep AK; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA. nmurthy@berkeley.edu.
  • Udenyi P; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA. nmurthy@berkeley.edu.
  • Han H; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Jackson N; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA. nmurthy@berkeley.edu.
  • Kerr DL; School of Public Health, Division of Infectious Diseases and Vaccinology, University of California Berkeley, Berkeley, CA, USA. lwriley@berkeley.edu.
  • Chen D; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Stentzel MR; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA. nmurthy@berkeley.edu.
  • Ruan E; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA. nmurthy@berkeley.edu.
  • Riley L; Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
  • Murthy N; School of Public Health, Division of Infectious Diseases and Vaccinology, University of California Berkeley, Berkeley, CA, USA. lwriley@berkeley.edu.
Chem Commun (Camb) ; 58(19): 3166-3169, 2022 Mar 03.
Article en En | MEDLINE | ID: mdl-35170593
This article reports the synthesis and characterization of a novel self-immolative linker, based on thiocarbonates, which releases a free thiol upon activation via enzymes. We demonstrate that thiocarbonate self-immolative linkers can be used to detect the enzymes penicillin G amidase (PGA) and nitroreductase (NTR) with high sensitivity using absorption spectroscopy. Paired with modern thiol amplification technology, the detection of PGA and NTR were achieved at concentrations of 160 nM and 52 nM respectively. In addition, the PGA probe was shown to be compatible with both biological thiols and enzymes present in cell lysates.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Penicilina Amidasa / Compuestos de Sulfhidrilo / Nitrorreductasas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Chem Commun (Camb) Asunto de la revista: QUIMICA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Penicilina Amidasa / Compuestos de Sulfhidrilo / Nitrorreductasas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Chem Commun (Camb) Asunto de la revista: QUIMICA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido