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A decontamination strategy for resolving SARS-CoV-2 amplicon contamination in a next-generation sequencing laboratory.
Mwangi, Peter; Mogotsi, Milton; Ogunbayo, Ayodeji; Mooko, Teboho; Maringa, Wairimu; Sondlane, Hlengiwe; Nkwadipo, Kelebogile; Adelabu, Olusesan; Bester, Philip Armand; Goedhals, Dominique; Nyaga, Martin.
Afiliación
  • Mwangi P; Next Generation Sequencing Unit and Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
  • Mogotsi M; Next Generation Sequencing Unit and Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
  • Ogunbayo A; Next Generation Sequencing Unit and Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
  • Mooko T; Next Generation Sequencing Unit and Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
  • Maringa W; Next Generation Sequencing Unit and Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
  • Sondlane H; Next Generation Sequencing Unit and Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
  • Nkwadipo K; Next Generation Sequencing Unit and Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
  • Adelabu O; Next Generation Sequencing Unit and Division of Virology, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa.
  • Bester PA; Division of Virology, National Health Laboratory Service and University of the Free State, Bloemfontein, South Africa.
  • Goedhals D; Division of Virology, National Health Laboratory Service and University of the Free State, Bloemfontein, South Africa.
  • Nyaga M; Pathcare Vermaak, Pretoria, 0157, South Africa.
Arch Virol ; 167(4): 1175-1179, 2022 Apr.
Article en En | MEDLINE | ID: mdl-35298714
ABSTRACT
Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) amplicon contamination was discovered due to next-generation sequencing (NGS) reads mapping in the negative controls. Environmental screening was undertaken to determine the source of contamination, which was suspected to be evaporation during polymerase chain reaction (PCR) assays while using the coronavirus disease 2019 (COVID-19) ARTIC protocol. A decontamination strategy is hereby documented to assist laboratories that may experience similar amplicon contamination. Routine molecular laboratory environmental screening as a quality control is highly recommended.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: COVID-19 / Laboratorios Límite: Humans Idioma: En Revista: Arch Virol Año: 2022 Tipo del documento: Article País de afiliación: Sudáfrica
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: COVID-19 / Laboratorios Límite: Humans Idioma: En Revista: Arch Virol Año: 2022 Tipo del documento: Article País de afiliación: Sudáfrica