Evaluation of the accuracy of multiplex polymerase chain reaction in differentiation between bacterial and viral meningitis.
Ir J Med Sci
; 192(1): 403-407, 2023 Feb.
Article
en En
| MEDLINE
| ID: mdl-35338445
ABSTRACT
BACKGROUND:
Meningitis is one of the most dangerous infection affecting children. The need for rapid and accurate diagnosis is mandatory for improving the outcome. AIM OF THE WORK To evaluate the role of multiplex polymerase chain reaction (PCR) in diagnosis of meningitis either bacterial or viral and to detect its accuracy. PATIENTS ANDMETHODS:
A cross-sectional study was carried out in University Children Hospital, Faculty of Medicine, between November 2019 and September 2020. The study was approved by the Ethics Review Board of Faculty of Medicine, Assiut University, and informed written consent was obtained. The committee's reference number is 17200161. Clinicaltrails.gov ID NCT03387969. Forty-eight children aged 2 to 18 years with meningitis were included. Detailed history and examination, blood glucose level at time of admission prior to lumbar puncture, and multiplex PCR in cerebrospinal fluid (CSF) were evaluated.RESULTS:
The mean age of children was 3.27 ± 1.27 years. Thirty-five (72.9%) cases were bacterial meningitis while 13 (27.1%) cases were viral meningitis. Multiplex PCR had 94% sensitivity and 100% specificity for diagnosis of bacterial meningitis.CONCLUSION:
Multiplex PCR may help in diagnosis and differentiation of bacterial and viral meningitis with accurate and rapid results.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Meningitis Bacterianas
/
Meningitis Viral
Tipo de estudio:
Observational_studies
Aspecto:
Ethics
Límite:
Child
/
Child, preschool
/
Humans
Idioma:
En
Revista:
Ir J Med Sci
Año:
2023
Tipo del documento:
Article
País de afiliación:
Egipto