More Insights from Ultrastructural and Functional Plasmodesmata Data Using PDinsight.

ABSTRACT

PDinsight is a Python-based tool for computing effective wall permeability for symplasmic transport based on plasmodesma (PD) size and distribution data. PDinsight can be used for direct computation of such permeabilities if full data is available, as well as in an explorative way if some data is either not available or considered unreliable. In this chapter, we briefly describe the basic model underlying the PDinsight calculations and discuss how the different modes of PDinsight can be used in relation to typical research questions. We also offer advice on choosing appropriate values for diffusion coefficients and particle size based on the currently most used experimental probes.