Isothermal Background-Free Nucleic Acid Quantification by a One-Pot Cas13a Assay Using Droplet Microfluidics.

Liu, Frank X; Cui, Johnson Q; Park, Hojeong; Chan, Ka Wai; Leung, Tyler; Tang, Ben Zhong; Yao, Shuhuai

Liu, Frank X; Cui, Johnson Q; Park, Hojeong; Chan, Ka Wai; Leung, Tyler; Tang, Ben Zhong; Yao, Shuhuai.

Afiliación

Liu FX; Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon 999077, Hong Kong.
Cui JQ; Department of Mechanical and Aerospace Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon 999077, Hong Kong.
Park H; Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon 999077, Hong Kong.
Chan KW; Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon 999077, Hong Kong.
Leung T; DiagCor Life Science Limited, Kowloon Bay, Kowloon 999077, Hong Kong.
Tang BZ; DiagCor Life Science Limited, Kowloon Bay, Kowloon 999077, Hong Kong.
Yao S; Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon 999077, Hong Kong.

Anal Chem ; 94(15): 5883-5892, 2022 04 19.

Article en En | MEDLINE | ID: mdl-35387453

Asunto(s)

Microfluídica; Ácidos Nucleicos; Bioensayo; Sistemas CRISPR-Cas; Técnicas de Amplificación de Ácido Nucleico/métodos; Recombinasas/metabolismo

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácidos Nucleicos / Microfluídica Idioma: En Revista: Anal Chem Año: 2022 Tipo del documento: Article País de afiliación: Hong Kong Pais de publicación: Estados Unidos

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