Novel Protein Production Method Combining Native Expression in Human Cells with an Intein-based Affinity Purification and Self-cleavable Tag.

Carman, Peter J; Dominguez, Roberto

Carman, Peter J; Dominguez, Roberto.

Afiliación

Carman PJ; Department of Physiology and Biochemistry and Molecular Biophysics Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Dominguez R; Department of Physiology and Biochemistry and Molecular Biophysics Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Bio Protoc ; 12(6): e4363, 2022 Mar 20.

Article en En | MEDLINE | ID: mdl-35434194

Palabras clave

Affinity purification; Expi293F cells; Intein; Post-translational Modification (PTM); Protein expression

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bio Protoc Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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