KIF14 affects cell cycle arrest and cell viability in cervical cancer by regulating the p27Kip1 pathway.
World J Surg Oncol
; 20(1): 125, 2022 Apr 19.
Article
en En
| MEDLINE
| ID: mdl-35439960
ABSTRACT
BACKGROUND:
Cervical cancer is a kind of malignant gynecological tumor. The first choice for treating cervical cancer is still a combination of surgery and chemoradiotherapy, but the 5-year survival rate remains poor. Therefore, researchers are trying to find new ways to diagnose and treat cervical cancer early.METHODS:
The expression level of KIF14 in cells and tissues was determined via qRT-PCR. The ability of the cells to proliferate, migrate, and invade was examined using CCK-8 assay kits, colony formation assays, and Transwell chambers. The expression levels of Cyclin D1, Cyclin B1, p21, and p27 were also detected using western blot assays.RESULTS:
The results suggested that p27 is a key regulatory factor in the KIF14-mediated regulation of the cell cycle. In addition, KIF14 knockdown promotes malignancy in cervical cancer cells by inhibiting p27 degradation, resulting in cell cycle arrest.CONCLUSIONS:
KIF14 is an oncogene in cervical cancer, and knocking down KIF14 causes cell cycle arrest by inhibiting p27 degradation, thus affecting cell viability, proliferation, and migration. These results provide a potential therapeutic target for cervical cancer.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias del Cuello Uterino
Límite:
Female
/
Humans
Idioma:
En
Revista:
World J Surg Oncol
Año:
2022
Tipo del documento:
Article
País de afiliación:
China