A deep-red fluorescent molecular rotor based on donor-two-acceptor modular system for imaging mitochondrial viscosity.

Yin, Xiaoxi; Cai, Yiping; Cai, Songtao; Jiao, Xiaojie; Liu, Chang; He, Song; Zeng, Xianshun

Yin, Xiaoxi; Cai, Yiping; Cai, Songtao; Jiao, Xiaojie; Liu, Chang; He, Song; Zeng, Xianshun.

Afiliación

Yin X; Tianjin Key Laboratory for Photoelectric Materials and Devices, Tianjin University of Technology Tianjin 300384 China hesong@tjut.edu.cn xshzeng@tjut.edu.cn +86-22-6021-6748.
Cai Y; Key Laboratory of Display Materials and Photoelectric Devices, Ministry of Education, School of Materials Science & Engineering, Tianjin University of Technology Tianjin 300384 China.
Cai S; Tianjin Key Laboratory for Photoelectric Materials and Devices, Tianjin University of Technology Tianjin 300384 China hesong@tjut.edu.cn xshzeng@tjut.edu.cn +86-22-6021-6748.
Jiao X; Key Laboratory of Display Materials and Photoelectric Devices, Ministry of Education, School of Materials Science & Engineering, Tianjin University of Technology Tianjin 300384 China.
Liu C; Tianjin Key Laboratory for Photoelectric Materials and Devices, Tianjin University of Technology Tianjin 300384 China hesong@tjut.edu.cn xshzeng@tjut.edu.cn +86-22-6021-6748.
He S; Key Laboratory of Display Materials and Photoelectric Devices, Ministry of Education, School of Materials Science & Engineering, Tianjin University of Technology Tianjin 300384 China.
Zeng X; Tianjin Key Laboratory for Photoelectric Materials and Devices, Tianjin University of Technology Tianjin 300384 China hesong@tjut.edu.cn xshzeng@tjut.edu.cn +86-22-6021-6748.

RSC Adv ; 10(51): 30825-30831, 2020 Aug 17.

Article en En | MEDLINE | ID: mdl-35516013

ABSTRACT

ABSTRACT

A new donor-two-acceptor modular fluorescence rotor DpCy7 involving a phenolate donor unit and two benzothiazolium acceptor moieties was designed and synthesized. The DpCy7 underwent an internal charge transfer to form a Cy7-like longer conjugated system fluorochrome at a physiological pH. The probe exhibited a strong turn-on (8.5-fold) deep-red emission with a larger Stokes shift in glycerol aqueous solutions with restriction of rotation. Both the fluorescence intensity and fluorescence lifetime displayed the linear relationship of viscosity changes in the logarithmic plots. Furthermore, the HeLa cell imaging experiments of DpCy7 indicated that the rotor could be used to monitor the mitochondrial viscosity in living cells. This new type of deep-red fluorescence rotor provides a potential platform for determining viscosity at subcellular levels.

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: RSC Adv Año: 2020 Tipo del documento: Article