Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk.
Food Chem X
; 14: 100305, 2022 Jun 30.
Article
en En
| MEDLINE
| ID: mdl-35520389
ABSTRACT
Two exopolysaccharides (EPS) producing strains, isolated from raw donkey milk were identified as Leuconostoc mesenteroides SL and Enterococcus viikkiensis N5 using 16S rDNA sequencing. The Box Benheken design exhibited the highest yield of EPS-SL (672.342 mg/L) produced by SL and of EPS-N5 (901 mg/L) produced by N5. The molecular weight was 1.68×104 for EPS-SL and 1.55×104 Da for EPS-N5. FTIR, NMR and GC-MS analysis showed that the EPS are heteropolysaccharides. The SEM image showed that the EPS-SL was smooth and represented a lotus leaf shape and EPS-N5 revealed a stiff-like, porous appearance and was more compact than EPS-SL. The TGA analyses showed high thermal stability and degradation temperature. Additionally, the two EPSs possessed antibacterial and antioxidant activity, and the EPS-SL had the stronger antioxidant activity. Consequently, these results suggest that the functional and biological properties of EPS-SL and EPS-N5 imply the potential application in the food and pharmaceutical industries.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Food Chem X
Año:
2022
Tipo del documento:
Article
País de afiliación:
Marruecos
Pais de publicación:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS