Structural characterization and anti-inflammatory activity of a pectin polysaccharide HBHP-3 from Houttuynia cordata.
Int J Biol Macromol
; 210: 161-171, 2022 Jun 15.
Article
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| MEDLINE
| ID: mdl-35533845
ABSTRACT
In this study, a hot buffer soluble Houttuynia cordata polysaccharide (HBHP-3) with a molecular weight of 397.4 kDa was isolated from H. cordata. HBHP-3 was composed of rhamnose, arabinose, glucose, galactose and galacturonic acid with molar ratio of 16.012.64.618.115.6. Structural analysis showed that the main chain of HBHP-3 was composed of â2)-α-L-Rhap-(1â, â4)-α-D-GalpA-(1â and â4)-ß-D-Galp-(1â. There were branched chains of α-L-Araf-(1â, â5)-α-L-Araf-(1â, â4)-α-D-Glcp-(1â, â6)-ß-D-Galp-(1â, ß-D-Galp-(1â connected to the O-4 positions of â2)-α-L-Rhap-(1â. HBHP-3 effectively inhibited the secretion of NO and the mRNA expression of pro-inflammatory cytokines in a dose-dependent manner in macrophages. HBHP-3 inhibited the phosphorylation of p65 and IκBα proteins as well, illustrating that HBHP-3 exerted its anti-inflammatory activity by inhibiting the activation of NF-κB pathway.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Houttuynia
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2022
Tipo del documento:
Article